You can do it, it helps to have a wheelstand especially when truing it. When you are truing it use only 8 spokes (one going each way on the four "sides" of the wheel) mark them with tape or something and leave all the rest loose. Once it's looking good gently tighten all the spokes, check the alignment, and finish tigtening them all. Pay attention to what the wheel is telling you when you are re assembling it, watch for indentations and anything that will clue you in to how the spokes were oriented before.